Application

Anti-DGCR8 Antibody, clone 11C10.1 is an antibody against DGCR8 for use in Western Blotting.

Research CategoryNeuroscience

Research Sub CategoryRNA Metabolism & Binding Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microprocessor complex subunit DGCR8 (UniProt Q8WYQ5; also known as DiGeorge syndrome critical region 8, Pasha) is encoded by the DGCR8 (also known as DGCRK6, LP4941) gene (ORF C22orf12; Gene ID 54487) in human. The DGCR8 gene encodes a double-stranded RNA-binding protein that functions as a non-catalytic subunit of the microprocessor complex which mediates the biogenesis of microRNAs (miRNA) from the primary microRNA (pri-microRNA) transcript. miRNAs are initially transcribed as pri-microRNA with a local stem-loop that is cleaved by the microprocessor complex to produce the precursor microRNA (pre-miRNA). The microprocessor complex is comprised of the RNase Drosha and the RNA-binding protein DGCR8. DGCR8 is reported to be phosphorylated by Erk and Abl, an event that promotes DGCR8 stability and stimulates Drosha recruitment and subsequent substrate pri-microRNAs processing.

Immunogen

Epitope: Region between WW and DRBM 1 domain.

GST-tagged recombinant fragment from the region between the WW and DRBM 1 domain of human DGCR8.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DGCR8 in 10 µg of HeLa cell lysate.

Specificity

Expected to react with spliced isoforms 1 and 3, but not isoform 2 of human DGCR8 reported by UniProt (Q8WYQ5).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed. 86.05 kDa (isoform 1) and 82.75 kDa (isoform 3). The detected band size is consistent with the reported apparent molecular weight (Ha, M., and Kim, V.N. (2014). Nat. Rev. Mol. Cell Biol. 15(8):509-524).